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discount PCR Gene Mutation Detection Kit
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# 14.01.2019 - 02:10:15
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Human ALDH2 gene polymorphism detection kit
◆Product Advantages
High specificity: the Taqman-MGB probe method can effectively improve
the specificity of genotyping.
High accuracy: paraffin seal sample, the whole process in a closed
tube, with β-actin internal control system, effectively prevent
product contamination and false negative.
High sensitivity: accurate classification in samples with low to 1ng/
L DNA can be obtained.
Simple and fast: the kit is easy to operate and the detection period
is short (1.5 hours), and the result is intuitive and reliable.
◆About ALDH2 genotyping
Provide nitroglycerin medication guidance and healthy alcohol
consumption advice.
Assist doctors in selecting the appropriate nitroglycerin dose or
changing to other medications to avoid drug resistance or adverse
effects.
Test once, benefit lifetime.
◆Nitroglycerin tolerance
Nitroglycerin ,which treats impaired cardiac function vasodilation as
it is converted to nitric oxide,is used worldwide for patients with
various ischemic and congestive cardiac diseases ,including angina
pectoris.
However ,its efficiency differs widely among individual .some reports
show that nitroglycerin has no effect in more than 25%of Asian people
because of the gene mutation.
Nitroglycerin tolerance is a result of inactivation of aldehyde
dehydrogenase2 (ALDH2).ALDH2 is the key enzyme for the
biotransformation from nitroglycerin into effective metabolite nitric
oxide(NO).A research ,conducted by Rui jin Hospital of Shanghai Jiao
Tong University and School of Science of Fudan University,found that
when ALDH2 rs671 mutation happens(Glu504lys),the enzyme activity of
ALDH2 decreases more than 10 times,which weakens its ability of
releasing NO [1]. Therefore ,ALDH2 genotyping is essential before
accepting nitroglycerin treatment.
◆ALDH2 and ADH1B:Key genes for alcohol metabolism
The ethanol detoxifying pathway in humans occurs mainly in the liver
and is carried out by two enzymatic steps .the primary enzymes
involved in alcohol metabolism are alcohol dehydrogenase (ADH) and
aldehyde dehydrogenase (ALDH).
ADH1B is one of the most impotant ADH genes ,and SNPs in ADH1B will
result in the production of enzymes with different activities .the
presence of ADH1B*2 allele would be associated with an increased
oxidizing capacity[2].
Ethanol metabolism occurs in two steps :ethanol is metabolized
quickly by alcohol dehydrogenase (ADH) to generate acetaldehyde.
Acetaldehyde is then metabolized by the mitochondrial dehydrogenase
2(ALDH2) to acetate.
ALDH2 is better knowfor its critical role in ethanol metabolism ,which
is probably the only ALDH enzyme that contributes significantly to
acetaldehyde metabolism .the ALDH2*2 carriers have a lower ALDH2
enzymatic activity and this deficiency is manifested by the
characteristic facial flushing ,headaches,nausea,dizziness,and cardiac
palpitations after consumption of alcoholic beverages[3].
◆Specifications
◆References
[1] Yifeng Li,Dandan Zhang,et al.Mitochondrial aldehyde dehydrogenase
2(ALDH2) Glu504Lys polymorphism contributes to the variation in
efficacy of sublingual nitroglycerin.J Clin Invest.2006
Feb;116(2):506-11.
[2]Peng GS,Yin SJ .Effect of the allelic variants of aldehyde
dehydrogenase ALDH2*2 and alcohol dehydrogenase ADH1B*2 on blood
acetaldehyde concentrations.Human Genomics. 2009 Jan;3(2):121-7.
[3]Quillen EE,Chen XD et al. aldh2 is associated to alcohol dependence
and is the major genetic determinant of “daily maximum drinks “ in a
GWAS study of an isolated rural Chinese sample.Am J Med Genet B
Neuropsychiatr Genet. 2014 Mar;165B(2).discount PCR Gene Mutation
Detection Kit
website:http://www.health-carebiotech.com/molecular-diagnostic-probe/pcr-gene-mutation-detection-kit/





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